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firefly luminometer
The Modulus™ Microplate Luminometer has a pre-programmed protocol for quick setup of the Dual-Luciferase® Reporter Assay. Results are reported in RLUs for each of the luciferase readings and in Ratio (firefly& ...
The mechanism of the luminometer is that it quantifies the amount of light generated by the reaction of the ATP with the firefly enzyme luciferase. The aggregate light produced is the same as the number of organisms existing in the sample.
Thus, the Dual-Glo® Assay can be used to measure both firefly and Renilla luminescence in multiwell plates using a plate-reading luminometer, CCD camera or similar imaging device without injectors. (Note: Injectors should& ...
ATP is measured through treating a sample containing ATP with a reagent that reacts with the ATP to produce light measured in a luminometer. Fireflies have this process occurring naturally which explains help of an enzyme catalyst luciferase& ...
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